5-FAM

2020-05-25 14:00:08      點(diǎn)擊:

5-FAM熒光試劑(fluorescence reagent)一種有機試劑,能與熒光較弱或不顯熒光的物質(zhì)共價(jià)或非共價(jià)結合形成熒光的配合物。如8-羥基喹啉、黃酮醇、石榴茜素R、桑色素等。在它們的分子中大多含有芳香環(huán)或雜環(huán)。主要用于無(wú)機元素的熒光測定,例如,鈹、鋁、硼、鎵、硒、鎂以及某些稀土元素。對有機化合物也能對它們所發(fā)的熒光起加強、穩定和提高選擇性的作用。


 5-FAM是一種可用作酸堿指示劑的熒光染料,5-FAM具有極強的濃度依賴(lài)性并且可以顯示較強的熒光,可通過(guò)羧基和氨基的反應標記蛋白質(zhì),核甘酸和多肽。


 5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.

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